• Question: What solvents do you believe would be most effective in changing HiPSC’s into islets of langerhans

    Asked by 645genp22 to Remsha, Oliver, Lisa, Fiana on 16 Nov 2018.
    • Photo: Remsha Afzal

      Remsha Afzal answered on 16 Nov 2018:

      Haha hi there 🙂 I am not a researcher into stem cells so I am not aware of the details of the protocols involved in differentiating them into specialized cells like islets of langerhaans. But here is a paper I found where they generated islets in cell culture from stem cells. This is what they mention ” This protocol takes 4–5 wk and involves a unique combination using factors that affect signaling in numerous pathways, including signaling by wnt, activin, hedgehog, EGF, TGFβ, thyroid hormone, and retinoic acid, as well as γ-secretase inhibition”. Here is a full link to the paper if you are interested : https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4617632/ 🙂